High Resolution EMs
   
By D. Despommier
     
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High Resolution Electron Micrographs
 
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  • You may use any of the images in this gallery but you MUST give credit to Dr. Dickson Despommier and trichinella.org.
 
Table of Contents
 
Introduction

The images presented in this gallery were collected over a 25-year period of time. Those familiar with the science and "black art" of electron microscopy know that the quality of an EM negative is dependent upon many things (i.e., the kind of knife - diamond or glass; fixative used; staining; the instrument used to view them, just to name a few). I began taking EMs on a Philips model 100 and ended up several years later working on the 300 series model by the same manufacturer. As fixative and staining techniques improved, so did the quality of my sections. What I present here are the "left-overs" from numerous studies, some of which were actually accepted for publication. This means that I have had at my disposal several hundred reasonably high quality images that up to now could not be seen by anyone else but myself. Life is too short to keep them under wraps, and so I offer them to the scientific community at-large for whatever purposes they may deem useful. Many of them could not have been considered for publication due to knife marks, stain artifacts, etc. Nonetheless, they may still prove useful to those with interests in the structures captured on these films. So, enjoy them, warts and all!

Each EM negative has been converted into a digital image using an Epson model 2200 scanner at 4,000 dpi. I have endeavored not to "fool" with them in Photoshop (as tempting as it was to cover up my ineptitude as an electron microscopist), including cropping or focusing by sharpening the image, so the viewer can have as unaltered a view of them as is possible, given the constraints of the medium. The only thing I have done is convert them to grayscale.

 

Trichinella spiralis Scanning EMs

6-hour-old
13-hour-old 17-hour-old male 24-hour-old male adult male adult male
           
Copulatory bell (high magnification) Copulatory bell Genitalia L1 Anus L2 Genitalia L4 Genitalia
           
L4 Genitalia L4 to adult molt Isolated Nurse cell-parasite complex Isolated Nurse cell-parasite complex Isolated Nurse cell-parasite complex Pepsin-HCl isolated L1
           
L1 Entering Cells (courtesy, K. Wright)          

 

Whole Worms

Adult worm with eggs Eggs and Newborn larva Eggs in situ mother and children Newborn larva Newborn larva
           
Newborn larva Newborn larva Pepsin-HCl isolated L1 Two newborn larvae and three eggs    

 

Adult Worms (Cross Section)

Adult (Low magnification) Adult (Low magnification) Adult stichocyte Adult stichocyte Adult stichocyte Adult stichocyte
           
Adult stichocyte Adult (High Magnification) Adult (High Magnification) Hypodermal gland cells Hypodermal gland cells Hypodermal gland cells L3-L4
           
Hypodermal gland cells L3-L4 Mother with children in utero        

 

Newborn Larvae

Newborn larva Newborn larva Newborn larva Newborn larva Newborn larva Newborn larva
           
Newborn larva Newborn larva Newborn larva Newborn larva Newborn larva Newborn larva
           
Newborn larva (high magnification) Newborn larva hindgut Newborn larva hindgut      

 

Alpha Stichocyte of the L1 Larva

Alpha 2 and Beta 2 granule Alpha 2 granule Alpha 2 granule Alpha 2 granule Alpha granules in situ Alpha stichocyte
           
Alpha stichocyte Alpha stichocyte Alpha stichocyte Alpha stichocyte Alpha stichocyte Alpha stichocyte
           
Alpha stichocyte Alpha stichocyte Alpha stichocyte Alpha stichocyte Alpha stichocyte Alpha stichocyte
           
Alpha stichocyte Alpha stichocyte Alpha stichocyte Alpha stichocyte Alpha stichocyte (high mag) Alpha stichocyte (low mag)
           
Alpha stichocyte (low mag) Alpha stichocyte (low mag) Alpha stichocyte with esophagus and duct Canalicular tree Day 18 alpha stichocyte (low mag) Golgi in Alpha stichocyte
           
Immature Alpha stichocyte Immature Alpha stichocyte        

 

Beta Stichocyte of the L1 Larva

Beta stichocyte (high mag) Beta 2 Secretory Granule Beta Stichocyte Beta Stichocyte Beta Stichocyte
           
Beta Stichocyte Beta Stichocyte Beta Stichocyte Beta Stichocyte Beta Stichocyte Beta Stichocyte
           
Beta Stichocyte Early Beta Stichocyte Early Beta Stichocyte      

 

Stichocyte (mixed types)

Alpha and beta stichocytes Alpha and beta stichocytes Alpha and beta stichocytes Alpha and beta stichocytes Alpha and beta stichocytes (high mag) Alpha and beta stichocytes (high mag)

 

Stichocyte in action

8 hr. old L1 in SI 2 high mag 8 hr. old L1 in SI 2 8 hr. old L1 in SI Beta stichocyte 8 hr. old L1 in SI larva in gut Beta stichocytes
           
         

 

L1 Larva Gut Track

L1 larva esophagus and duct from stichocyte L1 larva esophagus at the level of the nerve ring L1 larva esophagus L1 larva midgut L1 larva midgut  
           

 

Nurse cell-parasite interface

Day 4 post-infection Day 4 post-infection Day 4 post-infection Day 4 post-infection Day 4 post-infection Early Nurse cell-parasite compelx
           
Early Nurse cell-parasite compelx (high mag) Early Nurse cell-parasite compelx (high mag) Early Nurse cell-parasite compelx Mature Nurse cell-parasite Mature Nurse cell-parasite Mature Nurse cell-parasite
           
Mature Nurse cell-parasite Mature Nurse cell-parasite Mature Nurse cell-parasite Mature Nurse cell-parasite Mature Nurse cell-parasite Mature Nurse cell-parasite
           
Mature Nurse cell-parasite Mature Nurse cell-parasite Mature Nurse cell-parasite (high mag) Mature Nurse cell-parasite (high mag) Mature Nurse cell-parasite (high mag) Nurse cell-parasite
           
Nurse cell-parasite Nurse cell-parasite Nurse cell-parasite complex      

 

Nurse cell cytoplasm

Capsule formation Capsule formation Capsule formation Capsule formation Capsule formation Capsule formation
           
Capsule formation Capsule formation Early capsule formation Early capsule formation Early capsule formation Early capsule formation
           
Early capsule formation Early capsule formation Early capsule formation Early capsule formation Mature capsule Mature capsule
           
Mature capsule Mature Nurse cell Mature Nurse cell Mature Nurse cell Mature Nurse cell Mature Nurse cell
           
Mature Nurse cell Mature Nurse cell Mature Nurse cell Mature Nurse cell Mature Nurse cell Mature Nurse cell

 

Enlarged Nuclei in Nurse Cells

Mature Nurse cell enlarged nucleus Mature Nurse cell enlarged nucleus Mature Nurse cell enlarged nucleus Day 5 Nurse cell enlarged nucleus Day 5 Nurse cell enlarged nucleus  

 

Invading Cells

Invading cell Invading cell Invading cell Invading cell Invading cell Invading cell
           
Invading cell (low mag) Invading cell (high mag)        

 

Normal Muscle

       

 

1 Hour Parenteral Phase

       
           

 

Day 4 Parenteral Phase

Day 4 in situ Day 4 in situ Day 4 in situ Day 4 in situ Day 4 in situ Day 4 in situ
           
Day 4 in situ Day 4 in situ Day 4 in situ Day 4 in situ Day 4 in situ Day 4 Nursel Cell
           
Montage Day          

 

Development of Stichocytes L1

Day 12 Stichocyte Day 12 Stichocyte Day 12 Stichocyte Day 12 Stichocyte    

 

Miscellaneous

L1 nerve Molting worm Trypsin treated pepsin-HCl isolated L1 larva      
 
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