An immunoelectron micrograph of adult female
An immunoelectron micrograph of adult female worms including fetus and surrounding sheath, which are immunostained with anti phosphorylcholine monoclonal antibody, and secondary immunostained with anti mouseIgG antibody (biotin tagged). The immunoreaction is visualised by the third reaction with avidin -gold complex.
tissue adult female worms
1 st antibody anti phosphorylcholine monoclonal antibody
2nd antibody anti mouse IgG antibody (biotin tagged)
visual marker colloidal gold (avidin tagged)
The sheath is strongly positive by the immunostaining, suggesting the antigen is phosphorylcholin-related.
The fetus is negative by the immunostaining or very weakly positive if any.