An immunoelectron micrograph of muscle larvae

An immunoelectron micrograph of muscle larvae including the esophagus and surrounding tissues, which are immunostained with infected sera taken from Fischer rats 16weeks post infection with Trichinella spiralis, and secondary immunostained with anti rat IgG antibody (biotin tagged).  The immunoreaction is visualised by the third reaction with avidin -gold complex.

    tissue         muscle larvae
    1 st antibody    infected sera taken from Fischer rats
    2nd antibody    anti rat IgG antibody (biotin tagged)
    visual marker    colloidal gold   (avidin tagged)

The esophagus occupying substance is strongly positive by the immunostaining, suggesting the presence of antigen recognized by Fischer rats during the Trichinella infection.  The esophagus surrounding tissues ( basal lamina, hemolymph) are positive.