An immunoelectron micrograph of a muscle larva

An immunoelectron micrograph of a muscle larva including stichocyte granules (α-granules, β-granules), which is immunostained with anti TSL-1 monoclonal antibody, and secondary immunostained with anti rat IgG antibody (biotin tagged).  The immunoreaction is visualised by the third reaction with avidin -gold complex.

    tissue         muscle larva
    1 st antibody    anti TSL-1 monoclonal antibody 
    2nd antibody    anti rat IgG antibody (biotin tagged)
    visual marker    colloidal gold   (avidin tagged)

Positive staining is observed on α-granules, but notβ-granules near α-granules.