An immunoelectron micrograph of an adult
An immunoelectron micrograph of an adult worms including the body wall ( cuticle, hypodermis, hypodermal muscle) and the midgut with brush border (BB), which is immunostained with anti TSL-1 monoclonal antibody, and secondary immunostained with anti rat IgG antibody (biotin tagged). The immunoreaction is visualised by the third reaction with avidin -gold complex.
tissue adult worms
1 st antibody anti TSL-1 monoclonal antibody
2nd antibody anti rat IgG antibody (biotin tagged)
visual marker colloidal gold (avidin tagged)
The cuticles urface is negative by the immunostaining, whereas brush border is positive.
TSL-1 antigen is supposed to be stage specific, but not strictly stage specific.