An immunoelectron micrograph of an adult worms

An immunoelectron micrograph of an adult worms including the body wall ( cuticle, hypodermis, hypodermal muscle) and the midgut with brush border (BB), which is immunostained with anti TSL-1 monoclonal antibody, and secondary immunostained with anti rat IgG antibody (biotin tagged).  The immunoreaction is visualised by the third reaction with avidin -gold complex.

    tissue        adult worms
    1 st antibody    anti TSL-1 monoclonal antibody 
    2nd antibody    anti rat IgG antibody (biotin tagged)
    visual marker    colloidal gold   (avidin tagged)

The cuticles urface is negative by the immunostaining, whereas brush border is positive.
TSL-1 antigen is supposed to be stage specific, but not strictly stage specific.