An immunoelectron micrograph of a muscle larva

An immunoelectron micrograph of a muscle larva including the body wall (upper panel) and stichocyte granules (lower panel), which are immunostained with serum taken from a patient infected with Trichinella.
 The immunoreaction is visualised by the second reaction with protein A tagged colloidal gold.

    tissue         muscle larva
    1 st antibody    Trichinellosis patient serum
    2nd reaction    protein A tagged colloidal gold
    visual marker    colloidal gold  

The cuticle inner layer is positive by the immunostaining (upper panel), α−granules are positive (lower panel).